Bart Lambrecht & Hamida Hammad Unit - Immunoregulation and Mucosal Immunology

Research field:

  • Study of the molecular mechanism of DC-epithelial interaction in asthma
    and how this is influenced by infection and environment.
  • Study how the unfolded protein response interferes with the functioning
    of cells in the immune system and how this might lead to lung disease.

Group leaders:
Prof. Dr. Bart Lambrecht

Tel:+32 9 33 13 601  -  Fax:+32 9 221 76 73
E-mail: Bart.Lambrecht.spam.detractor@irc.vib-UGentspam.corruptor.be

& Prof. Hamida Hammad

Tel: +32 9 33 13 740  -  Fax:+32 9 221 76 73
E-mail: Hamida.Hammad.spam.detractor@irc.vib-UGentspam.corruptor.be


Research topic

Research in our group is focused on unraveling the functions of lung dendritic cells and epithelial cells in asthma and respiratory viral infection. Asthma is a chronic inflammatory disease of the airways characterized by eosinophilic airway inflammation, goblet cell metaplasia and bronchial hyperreactivity. A frequent cause of exacerbations is respiratory viral infection. There is an epidemic of asthma in the Western world, of which the cause in unclear, and for which novel forms of prevention and treatment are urgently needed. In allergic asthma, allergen-specific Th2 lymphocytes cause inflammation, as well as formation of allergen specific IgE, also known as allergic sensitization.

In the past years, our lab has found crucial roles for antigen presenting dendritic cells (DCs) in causing allergic sensitization. Using the logic of Koch’s postulates, we have shown that DCs also play a crucial role also in maintenance of chronic airway inflammation.
First, we have found increased numbers of activated DCs in the airways of humans and mice with asthma. Next, repeated administrations of allergen-exposed DCs to the airways led to all typical features of asthma and even to long term airway remodeling, characteristic of chronic asthma.
Finally, and most importantly, the removal of DCs from the airways of mice with chronic ongoing inflammation cures all the features of the disease. These studies showed that targeting airway DCs could be a novel therapeutic intervention strategy. However, our studies have also mentioned the crucial role of DCs in the clearance of respiratory viruses. In an attempt to find novel pathways to interfere with the activation of DCs, and thus to come up with new therapeutic interventions that selectively target DCs without putting patients at risk for infection, research in the group is currently focusing on the way airway DCs get activated to cause allergic disease. In this respect, all models have recently centered around the real-life allergen house dust mite (HDM). Inhalation of HDM can only lead to asthma when airway epithelial cells are triggered through TLR4 and release endogenous danger signals such as ATP or uric acid and pro-Th2 innate cytokines like IL1, IL25, IL33, GM-CSF and TSLP.
Moreover, it was shown that innate immune cells such as basophils or eosinophils would amplify Th2 responses initiated by DCs. Known environmental risk factors like cigarette smoke exposure and diesel exhaust particles promote HDM-driven asthma by triggering the function of DCs, epithelial cells or innate immune cells.

In recent work, we are unravelling how metabolism and the unfolded protein response intersect with the process of allergic sensitization and viral infection. The ultimate goal is to find novel treatment strategies that alter the long term course of asthma and can prevent or cure the disease.

 

Area of expertise

  • Dendritic and epithelial cell biology
  • Asthma and respiratory viral infection models in mice
  • Unfolded protein response in immune cells
  • Immunology

Technology transfer potential

  • Therapeutic targets in allergic asthma
  • Development of novel in vivo tools to monitor ER stress by non-invasive technologies


Selected publications

  1. Osorio F et al. The unfolded-protein-response sensor IRE-1α regulates the function of CD8α+ dendritic cells.
    Nature Immunology, 15, 248-57, 2014
  2. Plantinga M et al. Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen.
    Immunity, 38, 322-35, 2013
  3. Guilliams M et al. Alveolar macrophages develop from fetal monocytes that differentiate into long-lived cells in the first week of life via GM-CSF.
    Journal of Experimental Medicine, 210, 1977-92, 2013
  4. Kool M et al. An unexpected role for uric Acid as an inducer of T helper 2 cell immunity to inhaled antigens and inflammatory mediator of allergic asthma
    Immunity, 34, 527-40, 2011
  5. Kool M et al. The ubiquitin-editing protein a20 prevents dendritic cell activation, recognition of apoptotic cells, and systemic autoimmunity
    Immunity, 35, 82-96, 2011

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